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KMID : 0363319980190010431
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Journal of Korean Oriental Internal Medicine
1998 Volume.19 No. 1 p.431 ~ p.441
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Effect of Buthus on activity in cerebral synaptosomes
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Yoon Jong-Young
Shin Hyeon-Chul
Yoon Cheol-Ho
Seo Un-Kyo
Kim Jong-Ho
Jung Ji-Chun
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Abstract
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This study was undertaken to determine whether Buthus exract(BTE) affects activity of nervous tissues. The enzym activity was measured in synaptosomal fraction prepared from rabbit brain cortex. activity was inhibited by BTE over concentration range of 0.05-0.5% in a dose-dependent manner. The enzyme activity was increased by an increase in concentration from 5 to 100mM, concentration from 0.5 to 10mM, and concentration from 0.2 to 5mM. These changes in ion concentrations did not produce any effect on the inhibitory effect of BTE on activity. An increase in ATP concentration from 0.1 to 3mM caused an increase in the enzyme activity. The inhibition of the enzyme activity by BTE were not different between two ATP concentrations. A sulfhydryl group protector DTT prevented PCMB-induced inhibition of activity, but the BTE-induced inhibition was not altered by DTT. The inhibition of enzyme activity by combination of ouabain and BTE was not different from that by Buthus alone. These results suggest that Buthus exerts inhibitory effect on activity in cerebral synaptosomes, and the action mechansim is similar to that of ouabain.
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KEYWORD
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Buthus, ouabain
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